fluorescent celltracker green cmfda  (Yeasen Biotechnology)

Journal: Molecular Cancer

Article Title: piR-1170 drives brain metastasis and immune evasion via WTAP-mediated m6A methylation reprogramming in triple-negative breast cancer

doi: 10.1186/s12943-026-02568-y

Figure Lengend Snippet: Upregulated piR-1170 facilitates TNBC brain metastasis. A Schematic of the brain metastasis model establishment in BALB/c nude mice. B-C In vivo bioluminescence imaging ( B ) and corresponding fluorescence intensities ( C ) at 10 min, 14 days, 21 days and 28 days post-implantation ( n = 6 per group). D Representative images of HE staining in brain metastatic lesion formed by anta-NC and anta-1170 cells. E Tissues from the brain-metastatic lesion were used for IF staining to observe cancer cell-astrocyte interaction. Tumor cells, astrocyte cells and nuclei were stained with pan-CK (green), GFAP (red) or DAPI (blue), respectively. F Representative images of blood vessel densities in brain metastases formed by anta-NC and anta-1170 cells. Vascular endothelial cells and nuclei were stained with CD31 (red) or DAPI (blue), respectively. G-H In vivo bioluminescence imaging ( G ) and corresponding fluorescence intensities ( H ) at 0–72 h post-intracardiac injection ( n = 6 per group). I Tumor cell extravasation analysis at 48 h post-injection, showing CMFDA-labeled tumor cells (green), CD31 + vasculature (red), and DAPI + nuclei (blue), with quantitative extravasation data. J Quantitative analysis of tumor cell adhesion to astrocyte monolayers after alteration of piR-1170 in vitro. K Quantitative assessment of tumor cell adhesion to human brain microvascular endothelial cells (HBMECs) after alteration of piR-1170 in vitro. The error bars represent the SDs of triplicate experiments

Article Snippet: Then, the tops of the monolayers were seeded with cancer cells labeled with CellTracker Green CMFDA (Yeasen Biotech, Shanghai, China).

Techniques: In Vivo, Imaging, Fluorescence, Staining, Injection, Labeling, In Vitro

Journal: Molecular Cancer

Article Title: piR-1170 drives brain metastasis and immune evasion via WTAP-mediated m6A methylation reprogramming in triple-negative breast cancer

doi: 10.1186/s12943-026-02568-y

Figure Lengend Snippet: piR-1170 confers survival advantage in TNBC brain metastasis. A Schematic of intracranial injection-based brain metastasis model. B-C In vivo bioluminescence imaging ( B ) and corresponding fluorescence intensities ( C ) at 1 day, 3 days, 7 days and 11 days post-implantation ( n = 6 per group). D Representative HE staining images of metastatic foci in murine brain sections across experimental groups. E Representative bright-field images of brain metastasis-derived organoids from anta-NC and anta-1170 groups. F 3D confocal reconstruction of brain metastasis organoids stained with pan-CK (green, tumor cells) and Ki67 (red, proliferative cells). G Representative images and quantitation of HBMEC tube formation assay using conditioned media from experimental groups. H Schematic of in vitro blood-brain barrier (BBB) transendothelial migration assay. I Representative images (left) and statistical analysis (right) of migrated tumor cells in different groups. Tumor cells were labeled with CMFDA (green) and red arrows point to migrated tumor cells. J Representative images (left) and comparative analysis (right) of TNBC cell migratory capacity between groups. K Representative images (left) and quantitative analyses (right) of the migration area in the scratch wound assay. The error bars represent the SDs of triplicate experiments

Article Snippet: Then, the tops of the monolayers were seeded with cancer cells labeled with CellTracker Green CMFDA (Yeasen Biotech, Shanghai, China).

Techniques: Injection, In Vivo, Imaging, Fluorescence, Staining, Derivative Assay, Quantitation Assay, Tube Formation Assay, In Vitro, Migration, Labeling, Scratch Wound Assay Assay